Clark I Q, Orozco C, Cock I E, Clarke F M
Faculty of Science and Technology, Griffith University, Nathan, Brisbane, Australia.
J Reprod Fertil. 1994 Jan;100(1):279-89. doi: 10.1530/jrf.0.1000279.
The effect of ammonium sulfate on the capacity of sera of pregnant animals to induce the expression of increased rosette inhibition titres in the rosette inhibition assay, that is, to express the so-called early pregnancy factor activity, was reinvestigated. The results show that the sera of pregnant mice contain low molecular mass (less than 1 kDa) moieties active in the rosette inhibition assay. Some of these moieties could be removed from the macromolecular components of sera by dialysis; however, most, or at least the most potent, of these molecules were shown to be associated with macromolecular components of the sera and were not removed by dialysis. Treatment of sera of pregnant mice with 40% ammonium sulfate released the bound low molecular mass moieties and these moieties partitioned into the supernatant fraction, whereas the macromolecular components to which they were bound partitioned into the pellet fraction. Extensive dialysis removed the low molecular mass active moieties from the supernatant fraction. The macromolecular components remaining in the supernatant retentate fraction obtained after extensive dialysis counteracted the action of the low molecular mass moieties in a dose-dependent manner in inducing increased rosette inhibition titres. However, macromolecular components in the extensively dialysed pellet fraction associated with the low molecular mass moieties in the absence of ammonium sulfate and modified their dose-response characteristics in the biological assay.(ABSTRACT TRUNCATED AT 250 WORDS)
重新研究了硫酸铵对妊娠动物血清在玫瑰花结抑制试验中诱导玫瑰花结抑制效价升高表达能力的影响，即对所谓早期妊娠因子活性表达的影响。结果表明，妊娠小鼠血清中含有在玫瑰花结抑制试验中具有活性的低分子量（小于1 kDa）部分。其中一些部分可通过透析从血清的大分子成分中去除；然而，这些分子中的大多数，或至少是最有效的部分，被证明与血清的大分子成分相关，并且不能通过透析去除。用40%硫酸铵处理妊娠小鼠血清会释放结合的低分子量部分，这些部分会分配到上清液部分，而它们所结合的大分子成分会分配到沉淀部分。广泛透析可从上清液部分去除低分子量活性部分。在广泛透析后获得的上清液截留物部分中剩余的大分子成分，以剂量依赖的方式抵消了低分子量部分在诱导玫瑰花结抑制效价升高方面的作用。然而，在不存在硫酸铵的情况下，广泛透析的沉淀部分中的大分子成分与低分子量部分相关联，并在生物学试验中改变了它们的剂量反应特性。（摘要截断于250字）
