Palermo-Neto J, Flório J C, Sakate M
Applied Pharmacology and Toxicology Laboratory, School of Veterinary Medicine, University of São Paulo, Brasil.
Neurotoxicol Teratol. 1994 Jan-Feb;16(1):65-70. doi: 10.1016/0892-0362(94)90010-8.
The effects of prenatal amitraz exposure on physical and behavioral parameters of rats were studied. Pregnant rats were orally gavaged with amitraz (20 mg/Kg) or with distilled water (1.0 ml/Kg) on Days 1, 4, 7, 10, 13, 16, and 19 of pregnancy. After birth, cross-fostering was performed thus generating the following groups: control pups nursed by control dams (CC); control pups nursed by treated dams (CE); treated pups nursed by treated dams (EE) and treated pups nursed by control dams (EC). Results show that compared to pups of Group CC, groups prenatally exposed to amitraz (EC and EE) showed decreased age of vaginal opening. Group EE also showed earlier fur development and a delay in incisor eruption compared to Group CC. The ages of pinna detachment, eye and ear opening, testes descent, and reflex development (surface righting and startle) were not affected by amitraz exposure. Offspring of group CE also showed earlier fur development. Rats of group EE had higher locomotor activity and rearing frequency and shorter immobility time compared to the rats of group CC when observed in an open-field 30 days after birth but not 60 and 90 days. No significant differences were found in open-field behaviors among the CC, CE, and EC groups. The present findings indicate that prenatal exposure to amitraz caused transient developmental and behavioral changes in the exposed rat offspring and suggest that further investigation of the potential health risk of amitraz exposure to developing human offspring may be warranted.
研究了产前暴露于双甲脒对大鼠身体和行为参数的影响。在妊娠第1、4、7、10、13、16和19天，给怀孕大鼠经口灌胃双甲脒（20 mg/Kg）或蒸馏水（1.0 ml/Kg）。出生后，进行交叉寄养，从而产生以下几组：由对照母鼠哺育的对照幼崽（CC）；由经处理母鼠哺育的对照幼崽（CE）；由经处理母鼠哺育的经处理幼崽（EE）和由对照母鼠哺育的经处理幼崽（EC）。结果显示，与CC组幼崽相比，产前暴露于双甲脒的组（EC和EE）阴道开口年龄降低。与CC组相比，EE组还表现出毛发生长较早和门齿萌出延迟。耳廓分离、睁眼和开耳、睾丸下降以及反射发育（表面翻正和惊吓）的年龄不受双甲脒暴露的影响。CE组的后代也表现出毛发生长较早。出生后30天在旷场中观察时，与CC组大鼠相比，EE组大鼠具有更高的运动活性和竖身频率以及更短的不动时间，但在60天和90天时没有差异。CC、CE和EC组之间在旷场行为方面未发现显著差异。目前的研究结果表明，产前暴露于双甲脒会导致暴露的大鼠后代出现短暂的发育和行为变化，并表明可能有必要进一步研究双甲脒暴露对发育中的人类后代的潜在健康风险。
