Plisov S Iu, Merkulova T I, Shkapenko A L
Mol Biol (Mosk). 1994 Mar-Apr;28(2):407-12.
Glucocorticoid responsive element (GRE) located -252 to -209 bp upstream of the transcription initiation site of mouse metallothionein I gene (mMT I) was identified in transient gene transfer experiments. This GRE, on its own, was capable of enhancing a reporter CAT gene expression in response to dexamethasone in orientation-independent manner when linked cis to a heterologous HSV TK gene promoter. The duplication of the GRE showed a synergistic effect on dexamethasone induction of the CAT gene. Nevertheless, the GRE located in its natural promoter region of mMT I gene (-330 to +70 bp) was found to provide low if any glucocorticoid induction of linked CAT gene, while showing strong cadmium regulation, comparable to the in vivo level.
在瞬时基因转移实验中，确定了位于小鼠金属硫蛋白I基因（mMT I）转录起始位点上游-252至-209 bp处的糖皮质激素反应元件（GRE）。当该GRE顺式连接到异源单纯疱疹病毒胸苷激酶（HSV TK）基因启动子时，其自身能够以方向独立的方式响应地塞米松增强报告基因氯霉素乙酰转移酶（CAT）的表达。GRE的重复对CAT基因的地塞米松诱导显示出协同效应。然而，位于mMT I基因天然启动子区域（-330至+70 bp）的GRE被发现，即使有也只能对连接的CAT基因提供低水平的糖皮质激素诱导，同时显示出与体内水平相当的强镉调节作用。
