Gazina E V, Semiletov Iu A, Markova N V, Pavliuchenkova R P
Mol Gen Mikrobiol Virusol. 1994 Mar-Apr(2):21-4.
The plasmids have been constructed permitting expression of hepatitis B viral polymerase and its functional domains, the catalytic and end ones, as hybrid proteins containing 12 alien amino acids in the N-terminal. Immunoblotting with the rabbit antisera to N- and C-terminals of hepatitis B polymerase amino acid sequence has demonstrated that constructed plasmids determine the synthesis of the corresponding proteins in bacterial cells. HBV polymerase is processed in Escherichia coli cells. The successful expression of HBV polymerase and its functional domains makes possible the detailed study of this protein structure and function.
已经构建了质粒，可允许乙型肝炎病毒聚合酶及其功能结构域（催化结构域和末端结构域）作为在N端含有12个外源氨基酸的杂合蛋白进行表达。用针对乙型肝炎聚合酶氨基酸序列N端和C端的兔抗血清进行免疫印迹分析表明，构建的质粒可在细菌细胞中决定相应蛋白质的合成。乙型肝炎病毒聚合酶在大肠杆菌细胞中进行加工。乙型肝炎病毒聚合酶及其功能结构域的成功表达使得对该蛋白质的结构和功能进行详细研究成为可能。
