Cham B E, Knowles B R
J Lipid Res. 1976 Mar;17(2):176-81.
A technique has been developed which attains in 30 minutes complete removal of triglyceride, cholesterol, phospholipid, and unesterified fatty acids from plasma without protein denaturation. Plasma is agitated at room temperature with a mixture of butanol and di-isopropyl either in a 40:60 (v/v) ratio. The plasma proteins, including the apolipoproteins, remain in solution in the aqueous phase, while the organic phase contains the dissolved lipids. The phases can easily be separated by low speed centrifugation. Different lipids are simultaneously extracted, but the rate of extraction is most rapid for unesterified fatty acids, followed by triglyceride, cholesterol, and phospholipid at, respectively, decreasing rates. Selective extraction of unesterified fatty acids, triglyceride and total cholesterol can be achieved by di-isopropyl ether alone. Ionic strength and pH are not altered by these procedures.
已开发出一种技术，该技术可在30分钟内完全去除血浆中的甘油三酯、胆固醇、磷脂和未酯化脂肪酸，且不会使蛋白质变性。在室温下，将血浆与体积比为40:60的丁醇和二异丙醚混合物搅拌。包括载脂蛋白在内的血浆蛋白保留在水相溶液中，而有机相则含有溶解的脂质。通过低速离心可轻松分离各相。不同的脂质可同时被提取，但未酯化脂肪酸的提取速度最快，其次是甘油三酯、胆固醇和磷脂，提取速度依次降低。仅用二异丙醚即可实现未酯化脂肪酸、甘油三酯和总胆固醇的选择性提取。这些操作不会改变离子强度和pH值。
