Quon D V, Delgadillo M G, Khachi A, Smale S T, Johnson P J
Department of Microbiology and Immunology, University of California, School of Medicine, Los Angeles 90024-1747.
Proc Natl Acad Sci U S A. 1994 May 10;91(10):4579-83. doi: 10.1073/pnas.91.10.4579.
To identify regulatory elements that play a role in transcription initiation in ancient eukaryotes, we have analyzed the upstream regions of protein-coding genes from Trichomonas vaginalis, one of the most ancient eukaryotes studied to date. Characterization of seven protein-coding genes from this protist invariably revealed the presence of a highly conserved DNA sequence motif immediately upstream of the coding region. This 13-nt motif was shown to surround and contain precise sites for transcription initiation. No typical TATA boxes, positioned at 25-30 nt upstream of the transcription start sites of these genes, were found. The start-site regions from all seven T. vaginalis genes impart strong specific initiation of transcription in a mammalian in vitro transcription assay. This consensus promoter element in an ancient eukaryote is similar, both structurally and functionally, to initiator elements found in promoters of higher eukaryotes.
为了鉴定在古代真核生物转录起始过程中发挥作用的调控元件，我们分析了阴道毛滴虫（Trichomonas vaginalis）蛋白质编码基因的上游区域，阴道毛滴虫是迄今为止研究的最古老的真核生物之一。对这种原生生物的七个蛋白质编码基因进行表征，始终揭示出在编码区域上游紧邻处存在一个高度保守的DNA序列基序。这个13个核苷酸的基序被证明围绕并包含转录起始的精确位点。在这些基因转录起始位点上游25 - 30个核苷酸处未发现典型的TATA框。在哺乳动物体外转录试验中，所有七个阴道毛滴虫基因的起始位点区域都能强烈地特异性起始转录。这种古代真核生物中的共有启动子元件在结构和功能上与高等真核生物启动子中发现的起始子元件相似。
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