Thomashow L S, Milhausen M, Rutter W J, Agabian N
Cell. 1983 Jan;32(1):35-43. doi: 10.1016/0092-8674(83)90494-4.
We have isolated cDNA and genomic clones containing alpha- and beta-tubulin genes from Trypanosoma brucei. Each clone has been mapped, and the identity of the tubulin genes has been established by cross-hybridization with cloned chicken tubulin genes and by hybridization-selection and translation of trypanosome tubulin mRNA. In contrast with the dispersed organization of tubulin genes in other organisms, trypanosome alpha- and beta-tubulin genes are physically linked and clustered in tandem repeats of approximately 13-17 copies per haploid genome of alternating alpha- and beta-tubulin sequences.
我们已经从布氏锥虫中分离出了包含α-和β-微管蛋白基因的cDNA和基因组克隆。每个克隆都已进行定位，并且通过与克隆的鸡微管蛋白基因进行交叉杂交以及对锥虫微管蛋白mRNA进行杂交选择和翻译，确定了微管蛋白基因的身份。与其他生物中微管蛋白基因的分散组织不同，锥虫的α-和β-微管蛋白基因在物理上是相连的，并以串联重复的形式聚集，每个单倍体基因组中约有13 - 17个拷贝，α-和β-微管蛋白序列交替出现。
