Sakairi Y, Ando Y, Tabei K, Kusano E, Asano Y
Division of Nephrology, Jichi Medical School Hospital, Tochigi, Japan.
Am J Physiol. 1994 Apr;266(4 Pt 2):F674-80. doi: 10.1152/ajprenal.1994.266.4.F674.
Interleukin-1 (IL-1) induces natriuresis and diuresis. In the present study, the effect of basolateral IL-1 on sodium and water transport was examined in the cortical collecting duct (CCD) perfused in vitro. IL-1, 10 pg/ml and 10 ng/ml, inhibited lumen-to-bath sodium flux (JNa, peq.min-1.mm tubule-1), depolarizing transepithelial voltage (Vt) in a time- and dose-dependent manner. The inhibitory effect of 10 ng/ml but not 10 pg/ml IL-1 on Vt and JNa was mitigated by 5 microM indomethacin (IND) in bath. Also, 10 ng/ml IL-1, which did not affect the basal hydraulic conductivity (Lp, x10(-7) cm.atm-1.s-1) by itself, inhibited the hydrosmotic effect of 20 pM basolateral arginine vasopressin, and 5 microM IND abolished this inhibitory effect of 10 ng/ml IL-1. The present study demonstrated direct inhibitory effect of basolateral IL-1 on sodium and water reabsorption in the rabbit CCD. The effect of IL-1 is suggested to be mediated, in part, by a cyclooxygenase metabolite(s).
白细胞介素-1（IL-1）可诱导尿钠排泄和利尿。在本研究中，我们检测了体外灌流的皮质集合管（CCD）中基底外侧IL-1对钠和水转运的影响。10 pg/ml和10 ng/ml的IL-1以时间和剂量依赖的方式抑制管腔到浴液的钠通量（JNa，peq·min⁻¹·mm小管⁻¹），使跨上皮电压（Vt）去极化。浴液中5 μM的吲哚美辛（IND）可减轻10 ng/ml而非10 pg/ml的IL-1对Vt和JNa的抑制作用。此外，10 ng/ml的IL-1本身不影响基础水导率（Lp，x10⁻⁷ cm·atm⁻¹·s⁻¹），但可抑制20 pM基底外侧精氨酸加压素的渗透作用，5 μM的IND可消除10 ng/ml IL-1的这种抑制作用。本研究证明了基底外侧IL-1对兔CCD中钠和水重吸收具有直接抑制作用。提示IL-1的作用部分是由环氧化酶代谢产物介导的。
