Mulder M F, van Lambalgen A A, Huisman E, Visser J J, van den Bos G C, Thijs L G
Department of Clinical Chemistry, Free University Hospital, Amsterdam, The Netherlands.
Am J Physiol. 1994 Apr;266(4 Pt 2):H1558-64. doi: 10.1152/ajpheart.1994.266.4.H1558.
The role of NO during the first hour of endotoxemia is still controversial. To evaluate whether NO is protective or detrimental to the regulation of systemic blood pressure, cardiac output (CO), and organ perfusion in rats during acute endotoxemia, we have studied the effects of inhibition of NO synthesis. Thirty minutes after 0.1 mg NG-nitro-L-arginine (L-NNA; group L, n = 7, partial inhibition), 1 mg L-NNA (group H, n = 6, complete inhibition), or saline (group E, n = 7) intravenous infusion, anesthetized volume-loaded rats were infused with endotoxin Escherichia coli O127:B8 (8 mg.kg-1 x h-1) from time (t) = 0 to 60 min. Organ blood flow was measured with radioactive microspheres. In group H, at time 0, CO was lower than in group E (by -29%; P < 0.05), and systemic vascular resistance (SVR) was higher than in groups E and L (by 72 and 51%, respectively; P < 0.05). Perfusion of the pancreas, stomach, intestines, and kidney was lower (P < 0.05) and corresponding organ vascular resistance (OVR) higher (P < 0.05) in group H than in groups E and L (except kidney in group L). At t = 60 min, in groups H and L, CO was lower (by -45 and -26%, respectively; P < 0.05) and SVR was higher (by 112 and 54%, respectively; P < 0.05) than in group E. In group L only blood flow to the heart, pancreas, intestines, and kidney was significantly lower than in group E, and corresponding OVR was higher.(ABSTRACT TRUNCATED AT 250 WORDS)
内毒素血症最初一小时内一氧化氮（NO）的作用仍存在争议。为评估在急性内毒素血症期间NO对大鼠全身血压、心输出量（CO）及器官灌注调节是具有保护作用还是有害作用，我们研究了抑制NO合成的效果。在静脉输注0.1毫克NG-硝基-L-精氨酸（L-NNA；L组，n = 7，部分抑制）、1毫克L-NNA（H组，n = 6，完全抑制）或生理盐水（E组，n = 7）30分钟后，对麻醉且血容量充足的大鼠从时间（t）= 0至60分钟输注内毒素大肠杆菌O127:B8（8毫克·千克⁻¹·小时⁻¹）。用放射性微球测量器官血流量。在H组，时间为0时，CO低于E组（降低29%；P < 0.05），全身血管阻力（SVR）高于E组和L组（分别升高72%和51%；P < 0.05）。H组胰腺、胃、肠道和肾脏的灌注较低（P < 0.05），相应器官血管阻力（OVR）高于E组和L组（L组肾脏除外）。在t = 60分钟时，H组和L组的CO低于E组（分别降低45%和26%；P < 0.05），SVR高于E组（分别升高112%和54%；P < 0.05）。仅在L组，心脏、胰腺、肠道和肾脏的血流量显著低于E组，相应的OVR更高。（摘要截短至250字）
