Gao X, Jaffurs D, Robbins P D, Huang L
Department of Pharmacology, University of Pittsburgh, School of Medicine, PA 15261.
Biochem Biophys Res Commun. 1994 May 16;200(3):1201-6. doi: 10.1006/bbrc.1994.1578.
A plasmid containing the reporter gene, chloramphenicol acetyltransferase (CAT), driven by the bacteriophage T7 promoter was co-delivered with purified T7 RNA polymerase by the DC-chol cationic liposomes into human embryonic kidney 293 cells to obtain a transient (2 days) CAT gene expression. To prolong the expression, a T7 autogene which contains the T7 RNA polymerase gene driven by the T7 promoter was included in the transfection complex as a self-amplifying regeneration mechanism for the polymerase. High level CAT gene expression was observed up to 5 days after transfection. This strong and sustained expression system should be useful in gene transfer experiments.
