Chan Y L, Olvera J, Paz V, Wool I G
Department of Biochemistry and Molecular Biology, University of Chicago, Illinois 60637.
Biochem Biophys Res Commun. 1994 May 16;200(3):1498-504. doi: 10.1006/bbrc.1994.1620.
The amino acid sequence of the rat 40S ribosomal subunit protein S15a was deduced from the sequence of nucleotides in two recombinant cDNAs. Ribosomal protein S15a has 129 amino acids, the NH2-terminal methionine is removed after translation of the mRNA and has a molecular weight of 14,698. Hybridization of the cDNA to digests of nuclear DNA suggests that there are 10 to 15 copies of the S15a gene. The mRNA for the protein is about 650 nucleotides in length. Rat S15a is related to ribosomal proteins from other eukaryotes, from eubacteria, and from archaebacteria.
大鼠40S核糖体亚基蛋白S15a的氨基酸序列是根据两个重组cDNA中的核苷酸序列推导出来的。核糖体蛋白S15a有129个氨基酸，mRNA翻译后NH2末端的甲硫氨酸被去除，分子量为14,698。cDNA与核DNA消化产物的杂交表明，S15a基因有10到15个拷贝。该蛋白的mRNA长度约为650个核苷酸。大鼠S15a与来自其他真核生物、真细菌和古细菌的核糖体蛋白相关。
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