Charatjan S G, Wolnowa A I
Nahrung. 1975;19(9-10):885-90. doi: 10.1002/food.19750190921.
The microbiological analysis with the test-organism Tetrahymena pyriformis W can be used successfully to measure the relative nutritive value of the biomass and protein isolates from different yeasts for screening the groups of micro-organisms to choose those of the desired quality. In general, this method is rapid, comparatively cheap and gives the possibility to test large numbers of samples simultaneously. According to the literature and our own results, the relative nutritive value, the percentage of absorbed "N", retained by test-organism and used for its growth and multiplication expressed as cell count and given in relation to a standard protein (casein) is generally in a good agreement with results obtained with animals. This microbiological method is promising for investigating the influence of certain technological operations on the quality of protein. The investigation has been performed by the use of Baker's yeast Saccharomyces cerevisiae, of fodder yeast Candida, grown on oil hydrocarbons and on wood hydrolysates, yeasts Hansenula and Mycoderma, grown on a medium containing ethanol as the sole carbon source. The best results were obtained with the yeast grown on the ethanol-containing medium. The biological value of the biomass of yeast Hansenula is 91% and of protein isolate 96% as related to casein as the standard protein.
使用试验生物梨形四膜虫W进行微生物分析，可成功用于测定不同酵母的生物质和蛋白质分离物的相对营养价值，以筛选微生物群体，从而选择出具有所需质量的微生物。一般来说，该方法快速、成本相对较低，并且能够同时检测大量样品。根据文献和我们自己的结果，以标准蛋白质（酪蛋白）为参照，用试验生物吸收并用于其生长和繁殖（以细胞计数表示）的“N”的百分比来表示的相对营养价值，通常与用动物获得的结果高度一致。这种微生物学方法在研究某些工艺操作对蛋白质质量的影响方面很有前景。本研究使用了面包酵母酿酒酵母、以石油烃和木材水解物为培养基生长的饲料酵母假丝酵母、以含乙醇作为唯一碳源的培养基生长的汉逊酵母和假丝酵母。在以含乙醇培养基生长的酵母中获得了最佳结果。以酪蛋白作为标准蛋白质，汉逊酵母生物质的生物学价值为91%，蛋白质分离物的生物学价值为96%。
