Lea P J, Temkin R J, Freeman K B, Mitchell G A, Robinson B H
Canadian Genetic Diseases Network, Faculty of Medicine, University of Toronto, Ontario.
Microsc Res Tech. 1994 Mar 1;27(4):269-77. doi: 10.1002/jemt.1070270402.
Rat adrenal cortex was processed for high resolution scanning electron microscopy (HRSEM) to confirm tubular cristae, reported by transmission electron microscopy to be present in cortex mitochondria. Mitochondria in several other tissue and cell types were also observed and their ultrastructure confirmed by using three-dimensional, stereo, high resolution scanning electron microscopy. The mitochondria in rat and human hepatocytes as well as human skin fibroblasts grown in culture contained tubular cristae approximately 30 nanometers in diameter. The fibroblast mitochondria proved to be long, up to 46 micrometers and branching, as compared to those in liver which were spherical in shape. Cold adapted brown fat cells were packed with mitochondria, these containing plate or shelf-like cristae. Branched, rat striated muscle mitochondria were observed to curve around contractile protein filament bundles. The muscle mitochondrial cristae were found to be both tubular and plate-like, within the same mitochondrion. The ratio of tubular cristae to plate-like cristae varied considerably between muscle mitochondria. In order to use ultrastructural changes in mitochondria for differential diagnosis, and because 3D reconstruction of mitochondria based on transmission electron microscopy serial sections is severely limited in resolution, it is imperative to first develop a correct understanding of tissue specific, normal mitochondrial ultrastructure based on three-dimensional, HRSEM methods.
对大鼠肾上腺皮质进行处理，以用于高分辨率扫描电子显微镜（HRSEM）检查，从而确认透射电子显微镜报告的皮质线粒体中存在的管状嵴。还观察了其他几种组织和细胞类型中的线粒体，并使用三维、立体、高分辨率扫描电子显微镜确认了它们的超微结构。大鼠和人类肝细胞以及培养的人类皮肤成纤维细胞中的线粒体含有直径约30纳米的管状嵴。与肝脏中呈球形的线粒体相比，成纤维细胞线粒体被证明很长，长达46微米且有分支。冷适应的棕色脂肪细胞充满了线粒体，这些线粒体含有板状或架状嵴。观察到分支的大鼠横纹肌线粒体围绕收缩蛋白丝束弯曲。在同一线粒体中，发现肌肉线粒体嵴既有管状的也有板状的。肌肉线粒体中管状嵴与板状嵴的比例差异很大。为了利用线粒体的超微结构变化进行鉴别诊断，并且由于基于透射电子显微镜连续切片的线粒体三维重建在分辨率上受到严重限制，因此必须首先基于三维HRSEM方法正确理解组织特异性的正常线粒体超微结构。
