Bewick G S, Nicholson L V, Young C, Slater C R
Division of Neurobiology, University of Newcastle upon Tyne, U.K.
Neuromuscul Disord. 1993 Sep-Nov;3(5-6):503-6. doi: 10.1016/0960-8966(93)90105-s.
The relationship of a member of the transmembrane dystrophin-associated glycoprotein (DAG) complex to acetylcholine receptors (AChRs) was investigated using immunofluorescence techniques at rat neuromuscular junctions (NMJs) viewed en face. These results were compared with those from a similar previous study of dystrophin and an autosomal homologue (utrophin or dystrophin-related protein, DRP) (Bewick et al. Neuro Report 1992; 3: 857-860). The region of highest 43 K DAG (43DAG) labelling projected beyond the AChRs by approximately 0.3 microns, as does that for dystrophin. By contrast DRP labelling precisely co-localizes with the AChRs. These results suggest that at the NMJ, the region of high 43DAG concentration encompasses the area of highest intensity labelling for both DRP and dystrophin.
利用免疫荧光技术，在大鼠神经肌肉接头（NMJ）的正面视图中，研究了跨膜肌营养不良蛋白相关糖蛋白（DAG）复合物的一个成员与乙酰胆碱受体（AChR）之间的关系。将这些结果与之前一项关于肌营养不良蛋白和常染色体同源物（抗肌萎缩蛋白相关蛋白或肌营养不良蛋白相关蛋白，DRP）的类似研究结果进行了比较（Bewick等人，《神经报告》，1992年；3：857 - 860）。43K DAG（43DAG）标记最高的区域比AChR超出约0.3微米，肌营养不良蛋白的情况也是如此。相比之下，DRP标记与AChR精确共定位。这些结果表明，在NMJ处，高浓度43DAG区域包含了DRP和肌营养不良蛋白标记强度最高的区域。
