大鼠骨骼肌中肌营养不良蛋白相关糖蛋白与接头型乙酰胆碱受体簇的关系。
Relationship of a dystrophin-associated glycoprotein to junctional acetylcholine receptor clusters in rat skeletal muscle.
作者信息
Bewick G S, Nicholson L V, Young C, Slater C R
机构信息
Division of Neurobiology, University of Newcastle upon Tyne, U.K.
出版信息
Neuromuscul Disord. 1993 Sep-Nov;3(5-6):503-6. doi: 10.1016/0960-8966(93)90105-s.
The relationship of a member of the transmembrane dystrophin-associated glycoprotein (DAG) complex to acetylcholine receptors (AChRs) was investigated using immunofluorescence techniques at rat neuromuscular junctions (NMJs) viewed en face. These results were compared with those from a similar previous study of dystrophin and an autosomal homologue (utrophin or dystrophin-related protein, DRP) (Bewick et al. Neuro Report 1992; 3: 857-860). The region of highest 43 K DAG (43DAG) labelling projected beyond the AChRs by approximately 0.3 microns, as does that for dystrophin. By contrast DRP labelling precisely co-localizes with the AChRs. These results suggest that at the NMJ, the region of high 43DAG concentration encompasses the area of highest intensity labelling for both DRP and dystrophin.
利用免疫荧光技术，在大鼠神经肌肉接头（NMJ）的正面视图中，研究了跨膜肌营养不良蛋白相关糖蛋白（DAG）复合物的一个成员与乙酰胆碱受体（AChR）之间的关系。将这些结果与之前一项关于肌营养不良蛋白和常染色体同源物（抗肌萎缩蛋白相关蛋白或肌营养不良蛋白相关蛋白，DRP）的类似研究结果进行了比较（Bewick等人，《神经报告》，1992年；3：857 - 860）。43K DAG（43DAG）标记最高的区域比AChR超出约0.3微米，肌营养不良蛋白的情况也是如此。相比之下，DRP标记与AChR精确共定位。这些结果表明，在NMJ处，高浓度43DAG区域包含了DRP和肌营养不良蛋白标记强度最高的区域。
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