Casanovas M, Marañón F, Bel I
CBF-LETI S.A., Madrid, Spain.
Clin Exp Allergy. 1994 Feb;24(2):134-9. doi: 10.1111/j.1365-2222.1994.tb00209.x.
An in vitro technique for measuring the relative potency of allergenic extracts has been developed and compared with the dose-response skin-prick test. The in vitro technique is a modification of an ELISA based on the method of antigen capture using polystyrene microtitre wells as solid phase (REINA-competition) and is also based on a dose-response. Both methods have been applied to allergen extracts of Dermatophagoides pteronyssinus, Phleum pratense, Secale cereale, Lolium perenne, Plantago lanceolata, Artemisia vulgaris, Salsola kali and Parietaria judaica. For each allergen, the slope of the regression log-log dose-response lines displayed by skin-prick test and REINA-C does not show a statistically significant difference, being parallel (P < 0.001), and indicating that the allergen-ligand binding kinetics are identical in both methods.
一种用于测量变应原提取物相对效力的体外技术已被开发出来，并与剂量反应皮肤点刺试验进行了比较。该体外技术是基于使用聚苯乙烯微量滴定孔作为固相的抗原捕获方法（REINA竞争法）对酶联免疫吸附测定（ELISA）的一种改进，并且也是基于剂量反应。这两种方法都已应用于尘螨、梯牧草、黑麦、多年生黑麦草、长叶车前、普通蒿、盐角草和墙草的变应原提取物。对于每种变应原，皮肤点刺试验和REINA - C显示的回归对数 - 对数剂量反应线的斜率没有显示出统计学上的显著差异，呈平行关系（P < 0.001），这表明两种方法中变应原 - 配体结合动力学是相同的。
