Pendas A M, Moran P, Freije J P, Garcia-Vazquez E
Departamento de Biología Funcional, Facultad de Medicina, Oviedo, Spain.
Cytogenet Cell Genet. 1994;67(1):31-6. doi: 10.1159/000133792.
Atlantic salmon 5S ribosomal DNA (5S rDNA) was amplified by the polymerase chain reaction, using as primers conserved sequences from the coding region of rainbow trout 5S rRNA. Two amplified products of different molecular weights were obtained, cloned, and sequenced, revealing them to be tandemly arranged. The nucleotide sequences differed between the two clones in the length of the nontranscribed spacer (NTS) and in three nucleotides of the coding sequence. By means of fluorescence in situ hybridization the 5S rDNA was chromosomally located in the heterochromatic arm of the pair bearing the satellite, adjacent to the major ribosomal DNA locus (rDNA).
利用虹鳟5S核糖体RNA编码区的保守序列作为引物，通过聚合酶链反应扩增了大西洋鲑5S核糖体DNA（5S rDNA）。获得了两个不同分子量的扩增产物，将其克隆并测序，结果显示它们是串联排列的。两个克隆的核苷酸序列在非转录间隔区（NTS）的长度以及编码序列的三个核苷酸上存在差异。通过荧光原位杂交技术，5S rDNA被定位在携带卫星的染色体对的异染色质臂上，与主要核糖体DNA位点（rDNA）相邻。
