腺病毒5型/SV40整合位点与1号染色体1p36.1区域400 kb范围内U1小核RNA簇（RNU1）的同步定位。

Concurrent mapping of an adenovirus 5/SV40 integration site and the U1 snRNA cluster (RNU1) within 400 kb of the chromosome region 1p36.1.

作者信息

Romani M, Baldini A, Volpi E V, Casciano I, Nobile C, Muresu R, Siniscalco M

机构信息

Laboratory of Population Genetics, Istituto Nazionale per la Ricerca sul Cancro (IST), Alghero, Italy.

出版信息

Cytogenet Cell Genet. 1994;67(1):37-40. doi: 10.1159/000133793.

DOI:10.1159/000133793

PMID:8187549

Abstract

Previous reports from our group suggested the preferential integration of the viral construct Ad5/SV40 at the short arm subtelomeric region of human chromosome 1. The present study narrows the region of viral integration to site 1p36.1 in a close cytogenetic overlap with the U1 snRNA gene cluster (RNU1) within a distance necessarily smaller than 400 kb as suggested by the size of the YAC in which the two markers were found to coexist. This finding supports the hypothesis that the chromosomal site in question may have a constitutional propensity to genetic recombination.

摘要

我们团队之前的报告表明，病毒构建体Ad5/SV40优先整合于人染色体1短臂亚端粒区域。本研究将病毒整合区域缩小至1p36.1位点，该位点在细胞遗传学上与U1 snRNA基因簇（RNU1）紧密重叠，且距离必然小于400 kb，这是根据发现两个标记共存的YAC大小推断得出的。这一发现支持了这样的假设，即所讨论的染色体位点可能具有遗传重组的先天性倾向。