Oliver B, Kim Y J, Baker B S
Department of Biological Sciences, Stanford University, CA 94305.
Development. 1993 Nov;119(3):897-908. doi: 10.1242/dev.119.3.897.
Female sex determination in the germ line of Drosophila melanogaster is regulated by genes functioning in the soma as well as genes that function within the germ line. Genes known or suspected to be involved in germ-line sex determination in Drosophila melanogaster have been examined to determine if they are required upstream or downstream of Sex-lethal+, a known germ-line sex determination gene. Seven genes required for female-specific splicing of germ-line Sex-lethal+ pre-mRNA are identified. These results together with information about the tissues in which these genes function and whether they control sex determination and viability or just sex determination in the germ line have been used to deduce the genetic hierarchy regulating female germ-line sex determination. This hierarchy includes the somatic sex determination genes transformer+, transformer-2+ and doublesex+ (and by inference Sex-lethal+), which control a somatic signal required for female germ-line sex determination, and the germ-line ovarian tumor genes fused+, ovarian tumor+, ovo+, sans fille+, and Sex-lethal+, which are involved in either the reception or interpretation of this somatic sex determination signal. The fused+, ovarian tumor+, ovo+ and sans fille+ genes function upstream of Sex-lethal+ in the germ line.
黑腹果蝇生殖系中的雌性性别决定受躯体中发挥作用的基因以及生殖系内发挥作用的基因调控。已对已知或疑似参与黑腹果蝇生殖系性别决定的基因进行了研究，以确定它们是在已知的生殖系性别决定基因性致死基因（Sex-lethal+）的上游还是下游发挥作用。鉴定出了七个参与生殖系性致死基因前体信使核糖核酸（pre-mRNA）雌性特异性剪接所必需的基因。这些结果连同关于这些基因发挥作用的组织的信息，以及它们是控制性别决定和生存能力还是仅控制生殖系中的性别决定，已被用于推断调控雌性生殖系性别决定的遗传层次结构。这个层次结构包括躯体性别决定基因变异性转换基因（transformer+）、变异性转换基因-2（transformer-2+）和双性基因（doublesex+）（由此推断还有性致死基因），它们控制雌性生殖系性别决定所需的躯体信号，以及生殖系卵巢肿瘤基因融合基因（fused+）、卵巢肿瘤基因（ovarian tumor+）、卵母细胞基因（ovo+）、无女儿基因（sans fille+）和性致死基因，它们参与该躯体性别决定信号的接收或解读。融合基因、卵巢肿瘤基因、卵母细胞基因和无女儿基因在生殖系中于性致死基因的上游发挥作用。
