Kontermann R E, Bautz E K
Institute of Molecular Genetics, University of Heidelberg, Germany.
FEBS Lett. 1994 May 16;344(2-3):166-70. doi: 10.1016/0014-5793(94)00380-7.
Analysing overlapping bacterially expressed fragments of the second-largest subunit of Drosophila melanogaster RNA polymerase II in Southwestern DNA binding assays we have identified regions that have the potential to bind nucleic acids non-specifically. A region exhibiting strong DNA binding is located in the N-terminal part of the molecule (amino acids 357-504) and some weak DNA binding is observed for the C-terminal part (amino acids 860-1160). The non-specific DNA binding behavior of these regions is similar to that of the native enzyme. Most of the known mutations responsible for rifampicin resistance map to a region of the Escherichia coli beta subunit corresponding to the N-terminal nucleic acid-binding region, indirectly supporting the notion that this region participates in interaction with the RNA transcript in ternary complexes.
在西南DNA结合试验中，我们分析了果蝇RNA聚合酶II第二大亚基的重叠细菌表达片段，确定了有可能非特异性结合核酸的区域。一个表现出强DNA结合能力的区域位于分子的N端部分（氨基酸357 - 504），而在C端部分（氨基酸860 - 1160）观察到一些弱DNA结合。这些区域的非特异性DNA结合行为与天然酶相似。大多数已知的利福平抗性突变定位在大肠杆菌β亚基中与N端核酸结合区域相对应的一个区域，这间接支持了该区域参与三元复合物中与RNA转录本相互作用的观点。
