从格雷夫斯病患者中分离甲状腺过氧化物酶并与动物过氧化物酶进行比较。

Isolation of thyroid peroxidase from patients with Graves' disease and comparison with animal peroxidases.

Hosoya T, Yoshimura H, Naito N, Suzuki N, Sato K, Takamatsu J

Faculty of Pharmaceutical Sciences, Chiba University, Japan.

Int J Biochem. 1994 Mar;26(3):351-65. doi: 10.1016/0020-711x(94)90055-8.

Abstract

Human thyroid peroxidase (TPO) was isolated from 280-640 g of pooled thyroid tissue resected from patients with Graves' disease. 2. Isolation was performed by an improved and simplified method. 3. The Reinheit Zahl (A412/A280) of the final preparations was in the range of 0.16-0.32. 4. The spectroscopic and enzymatic properties of Graves' TPO were compared with those of porcine TPO and bovine LPO, revealing closer resemblance to the former. 5. Graves' TPO may provide a useful substitute for normal TPO, which is very difficult to isolate.

摘要

人甲状腺过氧化物酶（TPO）是从280 - 640克取自格雷夫斯病患者的合并甲状腺组织中分离出来的。2. 分离采用了一种改进和简化的方法。3. 最终制剂的比吸光系数（A412/A280）在0.16 - 0.32范围内。4. 将格雷夫斯病TPO的光谱和酶学性质与猪TPO和牛LPO的性质进行了比较，发现其与前者更为相似。5. 格雷夫斯病TPO可能是难以分离的正常TPO的有用替代品。