大鼠肝脏内质网蛋白激酶

Rat liver endoplasmic reticulum protein kinases.

Kosmopoulou I, Koliakos G, Haitoglou C, Christodoulou D, Dimitriadou A, Trakatellis A

Department of Biological Chemistry, Medical School, Aristotelian University of Thessaloniki, Greece.

Int J Biochem. 1994 Mar;26(3):403-14. doi: 10.1016/0020-711x(94)90061-2.

Abstract

Rat liver microsomal membranes were studied for the presence of protein kinases. Microsomal proteins solubilized with Triton X-100 were analyzed by means of ion exchange chromatography. 2. Protein kinase activity was detected in the column fractions using specific assays for cAMP-dependent protein kinase, cGMP-dependent protein kinase, protein kinase C, Ca2+/calmodulin-dependent protein kinase and casein kinases. 3. Fractions with protein kinase activity were further analyzed by SDS-polyacrylamide gel electrophoresis. 4. The results indicate that cAMP-dependent protein kinase type I and II, casein kinases I and II, protein kinase C proenzymes I and II and Ca2+/calmodulin kinase II are associated with the membranes of endoplasmic reticulum (ER).

摘要

对大鼠肝微粒体膜进行了蛋白激酶存在情况的研究。用离子交换色谱法分析了用Triton X-100溶解的微粒体蛋白。2. 使用针对环磷酸腺苷（cAMP）依赖性蛋白激酶、环磷酸鸟苷（cGMP）依赖性蛋白激酶、蛋白激酶C、钙离子/钙调蛋白依赖性蛋白激酶和酪蛋白激酶的特异性检测方法，检测柱层析馏分中的蛋白激酶活性。3. 对具有蛋白激酶活性的馏分进一步进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-聚丙烯酰胺凝胶电泳）分析。4. 结果表明，I型和II型cAMP依赖性蛋白激酶、酪蛋白激酶I和II、蛋白激酶C原酶I和II以及钙离子/钙调蛋白激酶II与内质网（ER）膜相关。