Trenina M A, Akhverdian V Z, Krylov V N
Genetika. 1994 Jan;30(1):54-6.
A clone of Escherichia coli II-16 with unique properties was isolated upon incorporation of hybrid plasmid RP4::D3112 with an integrated genome of phage-transposon D3112 Pseudomonas aeruginosa into E. coli C600 cells. The cells of this clone produce viable phage and are not sensitive to growth under low temperatures, which is characteristic of the majority of E. coli (RP4::D3112) clones with the genome of wild type phage. The clone E. coli II-16 contains phage genome both in an integrated state within the chromosome and in plasmid RP4. The properties of phage D3112 in the clone II-16 demonstrated that the phage carried a mutation. The mutation was designated RP4-phage interaction (rpi). The phenotypic effect of this mutation is expressed as phage inability to replicate in response to the presence of plasmid RP4 at 30 C (Tcs phenotype). The mutant rpi differs in its characters from the previously described mutants in the early regulator gene cip, the analog of the ner gene of E. coli phage Mu1, and from the known mutations in the A gene. Plasmid RP4::D3112 rpi exerts an inhibitory effect on the burst size of RP4::D3112 in E. coli.
将带有整合了铜绿假单胞菌噬菌体转座子D3112基因组的杂交质粒RP4::D3112导入大肠杆菌C600细胞后，分离得到了具有独特特性的大肠杆菌II-16克隆。该克隆的细胞可产生有活力的噬菌体，并且在低温下仍能生长，这与大多数带有野生型噬菌体基因组的大肠杆菌（RP4::D3112）克隆不同。大肠杆菌II-16克隆的染色体和质粒RP4中均含有噬菌体基因组。克隆II-16中噬菌体D3112的特性表明该噬菌体发生了突变。此突变被命名为RP4-噬菌体相互作用（rpi）。该突变的表型效应表现为在30℃时，噬菌体在质粒RP4存在的情况下无法复制（Tcs表型）。突变体rpi在特性上与先前描述的早期调节基因cip（大肠杆菌噬菌体Mu1的ner基因类似物）中的突变体以及A基因中的已知突变不同。质粒RP4::D3112 rpi对大肠杆菌中RP4::D3112的爆发量具有抑制作用。
