131I-去唾液酸α1-酸性糖蛋白的制备
Preparation of 131I-asialo-alpha1-acid glycoprotein.
作者信息
van Rijk P P, Heinsius H L, van den Hamer C J
出版信息
Vox Sang. 1976;30(6):412-9. doi: 10.1111/j.1423-0410.1976.tb02846.x.
Alpha1-Acid glycoprotein (orosomucoid) was prepared from a byproduct of the ethanol plasma fractionation by means of ion-exchange procedures. Immunoelectrophoresis suggested a high degree of purity; the purified protein contained 13.5% sialic acid and 17.8% hexose. The alpha1-acid glycoprotein was modified by removal of sialic acid with neuraminidase (EC 3.2.1.18) followed by iodination with 131I. The purpose of the preparation, its potential use as a pharmacon for liver function studies in nuclear medicine is the subject of further study.
α1-酸性糖蛋白（血清类黏蛋白）是通过离子交换程序从乙醇血浆分级分离的副产物中制备的。免疫电泳显示其纯度很高；纯化后的蛋白质含有13.5%的唾液酸和17.8%的己糖。先用神经氨酸酶（EC 3.2.1.18）去除唾液酸，然后用131I进行碘化，对α1-酸性糖蛋白进行修饰。该制剂的制备目的，即其作为核医学肝功能研究药物的潜在用途，是进一步研究的主题。
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