Kuramoto T, Maihara T, Masu M, Nakanishi S, Serikawa T
Institute of Laboratory Animals, Faculty of Medicine, Kyoto University, Japan.
Genomics. 1994 Jan 15;19(2):358-61. doi: 10.1006/geno.1994.1069.
Five N-methyl-D-aspartate receptor subunit genes and six metabotropic glutamate receptor subtype genes have been assigned to particular rat chromosomes by using a rat x mouse somatic cell hybrid clone panel. N-Methyl-D-aspartate receptor subunit genes (gene symbol, GRIN) GRIN1, GRIN2A, GRIN2B, GRIN2C, and GRIN2D have been assigned to chromosomes (Chr) 3, 10, 4, 10, and 1, respectively. Metabotropic glutamate receptor subtype genes (gene symbol, GRM) GRM1, GRM2, GRM3, GRM4, GRM5, and GRM6 have been assigned to Chr 1, 8, 4, 20, 1, and 10, respectively. In addition, GRIN2A and GRM6 loci were successfully localized on Chr 10 linkage maps by linkage analyses. The genetic distances between loci in cM (+/- SD) are as follows: GRIN2A-28.6(+/- 7.0)-RR24-23.3(+/- 6.4)-MYHSE, from a linkage analysis using the (SHR x WTC)F1 x WTC cross, and RR24-4.2(+/- 2.9)-GRM6-4.2(+/- 2.9)-MMYHSE-2.1(+/- 2.1)-ASGR, SHBG-27.1(+/- 6.4)-PPY, from a linkage analysis using the (ZI x TM)F1 x ZI cross.
通过使用大鼠×小鼠体细胞杂交克隆板，已将五个N-甲基-D-天冬氨酸受体亚基基因和六个代谢型谷氨酸受体亚型基因定位到特定的大鼠染色体上。N-甲基-D-天冬氨酸受体亚基基因（基因符号，GRIN）GRIN1、GRIN2A、GRIN2B、GRIN2C和GRIN2D已分别定位到染色体（Chr）3、10、4、10和1上。代谢型谷氨酸受体亚型基因（基因符号，GRM）GRM1、GRM2、GRM3、GRM4、GRM5和GRM6已分别定位到Chr 1、8、4、20、1和10上。此外，通过连锁分析，GRIN2A和GRM6基因座成功定位到Chr 10连锁图谱上。基因座之间以厘摩（cM）（±标准差）表示的遗传距离如下：使用（SHR×WTC）F1×WTC杂交进行连锁分析得到，GRIN2A-28.6（±7.0）-RR24-23.3（±6.4）-MYHSE；使用（ZI×TM）F1×ZI杂交进行连锁分析得到，RR24-4.2（±2.9）-GRM6-4.2（±2.9）-MMYHSE-2.1（±2.1）-ASGR，SHBG-27.1（±6.4）-PPY。
