Honiger J, Darquy S, Reach G, Muscat E, Thomas M, Collier C
INSERM Bioengineering Laboratories, Hôpital Saint-Antoine, Paris, France.
Int J Artif Organs. 1994 Jan;17(1):46-52.
The occurrence of an inflammatory reaction represents the major obstacle to the development of any implantable system including micro and macroencapsulation. The purpose of this study was to describe an encapsulation method for cells using a membrane made of AN69, a copolymer of acrylonitrile which is considered as a reference in biocompatibility in the field of haemodialysis. The hydrogel of AN69 was obtained after a coagulation step at room temperature followed by a solvent/non-solvent (water) exchange phase. Microcapsules were obtained by co-extrusion of AN69 collodion and saline (with or without cells). The function of encapsulated cells was assessed in vitro, demonstrating cell survival after the microencapsulation procedure. These preliminary data are consistent with the potential interest for the development of the microencapsulation procedure aimed at realising a bioartificial pancreas.
炎症反应的发生是包括微囊化和大囊化在内的任何可植入系统发展的主要障碍。本研究的目的是描述一种使用由AN69制成的膜对细胞进行包封的方法，AN69是一种丙烯腈共聚物，在血液透析领域被视为生物相容性的参考材料。AN69水凝胶是在室温下经过凝固步骤，随后进行溶剂/非溶剂（水）交换阶段后获得的。微胶囊通过将AN69火棉胶与盐水（含或不含细胞）共挤出获得。在体外评估了包封细胞的功能，证明了微囊化过程后细胞的存活。这些初步数据与旨在实现生物人工胰腺的微囊化程序开发的潜在意义相一致。
