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An antibody for phosphorylated myosin light chain of smooth muscle: application to a biochemical study.

作者信息

Sakurada K, Ikuhara T, Seto M, Sasaki Y

机构信息

Life Science Center, Asahi Chem. Indust. Co., Ltd., Miyazaki.

出版信息

J Biochem. 1994 Jan;115(1):18-21. doi: 10.1093/oxfordjournals.jbchem.a124297.

DOI:10.1093/oxfordjournals.jbchem.a124297
PMID:8188629
Abstract

An antibody with specificity for the 20 kDa myosin light chain of smooth muscle phosphorylated by myosin light chain kinase was developed. The antibody was raised against the phosphorylated synthetic peptide, Lys-Lys-Arg-Pro-Gln-Arg-Ala-Thr-phospho-Ser-Asn-Val-Phe-Cys (residues 11-22 of the myosin light chain). This antibody only recognized the monophosphorylated myosin light chain at serine 19, i.e., with no detectable recognition of nonphosphorylated or diphosphorylated serine 19 or threonine 18. The specificity was tested by EIA assaying of myosin light chain kinase activity using a 96-well plate coated with the light chain. This EIA system was as sensitive as the radioisotopic method, and the newly developed non-radioisotopic method.

摘要

相似文献

1
An antibody for phosphorylated myosin light chain of smooth muscle: application to a biochemical study.
J Biochem. 1994 Jan;115(1):18-21. doi: 10.1093/oxfordjournals.jbchem.a124297.
2
Sites phosphorylated in myosin light chain in contracting smooth muscle.收缩性平滑肌中肌球蛋白轻链发生磷酸化的位点。
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Hydroxyamino acid specificity of smooth muscle myosin light chain kinase.平滑肌肌球蛋白轻链激酶的羟基氨基酸特异性
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