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通过蔗糖梯度离心法分离脑膜炎奈瑟菌感受态变体。

Separation of Neisseria meningitidis competence variants by sucrose gradient centrifugation.

作者信息

Jyssum K

出版信息

Acta Pathol Microbiol Scand B. 1976 Apr;84(2):85-92. doi: 10.1111/j.1699-0463.1976.tb01907.x.

DOI:10.1111/j.1699-0463.1976.tb01907.x
PMID:818874
Abstract

Transformable (cp+) and non transformable (cp-) variants of the Neisseria meningitidis Strain M1 could be separated on the basis of their sedimentation velocity in sucrose gradients. The cp+ cells sedimented slightly more slowly than the cp- ones. This supports previous observations that the change from cp+ to cp- is followed by alterations in cell structures. In cp+ populations, the transformable fraction banded with the bulk of the cells in sucrose gradients, indicating that the density of the phenotypically competent cell is identical to the rest of the cp+ population.

摘要

脑膜炎奈瑟菌菌株M1的可转化(cp+)和不可转化(cp-)变体可以根据它们在蔗糖梯度中的沉降速度来分离。cp+细胞的沉降速度比cp-细胞略慢。这支持了先前的观察结果,即从cp+到cp-的转变伴随着细胞结构的改变。在cp+群体中,可转化部分在蔗糖梯度中与大部分细胞一起形成条带,表明表型有能力的细胞的密度与cp+群体的其他细胞相同。

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