Oguchi S, Yamada S, Oguchi H, Nakane P K
2nd Department of Internal Medicine, Shinshu University School of Medicine, Matsumoto, Japan.
J Clin Lab Anal. 1994;8(2):99-104. doi: 10.1002/jcla.1860080208.
Masugi nephritis was induced in rats by the administration of nephrotoxic duck serum and, in their kidneys, the distributions of transforming growth factor-beta 1 (TGF-beta 1) mRNA, TGF-beta 1, and macrophage antigenic marker were investigated. Kidney specimens were obtained at 3, 7, and 14 days after the injection. By in situ hybridization, TGF-beta 1 mRNA was found to be localized in the cells of mesangial region from the early stage of nephritis. By the immunohistochemical stainings, TGF-beta 1 was found to be localized in the cells of distal convoluted tubulus throughout the experiments and the macrophage antigenic marker was found to be localized in the occasional cells which were different from that contained TGF-beta 1 mRNA. Our data indicate that TGF-beta 1 mRNA is expressed by the resident glomerular cells and not by the migrating macrophages in rat kidney with Masugi nephritis.
通过给大鼠注射肾毒性鸭血清诱导Masugi肾炎，并研究其肾脏中转化生长因子β1（TGF-β1）mRNA、TGF-β1和巨噬细胞抗原标志物的分布。在注射后3天、7天和14天获取肾脏标本。通过原位杂交发现，从肾炎早期开始，TGF-β1 mRNA就定位于系膜区细胞。通过免疫组织化学染色发现，在整个实验过程中TGF-β1定位于远曲小管细胞，而巨噬细胞抗原标志物定位于偶尔出现的与含有TGF-β1 mRNA的细胞不同的细胞中。我们的数据表明，在患有Masugi肾炎的大鼠肾脏中，TGF-β1 mRNA由肾小球固有细胞表达，而非迁移的巨噬细胞表达。
