Chackerian B, Morton W R, Overbaugh J
Department of Microbiology, University of Washington, Seattle 98195.
J Virol. 1994 Jun;68(6):4080-5. doi: 10.1128/JVI.68.6.4080-4085.1994.
In macaques infected with a clone of simian immunodeficiency virus (SIV) Mne, viral variants consistently evolve multiple new potential glycosylation sites in the first variable region (V1) prior to the development of AIDS. In the present study, we asked whether viruses with these glycosylation sites persist when they are transmitted to a naive macaque. Variants that evolved after transmission to a recipient macaque were compared with virus that evolved in the donor, which had been infected by cloned SIV Mne. Upon transmission, the specific serine/threonine-rich motifs potentially encoding novel O-linked glycosylation site(s) in V1 were conserved in virus isolated from lymph node, spleen, and liver tissue from the recipient. There was some accumulation of changes in V3 of envelope in virus from the recipient, whereas changes in this region were not observed in virus from the donor macaque. Some variants detected in the tissue of the recipient at necropsy were most closely related to viruses present in the donor inoculum even though these particular variants were not detected early after infection in the recipient's peripheral blood mononuclear cells. Overall, virus with the predominant V1 sequences associated with progression to disease are transmitted to and persist in the recipient animal.
在感染猿猴免疫缺陷病毒（SIV）Mne克隆株的猕猴中，病毒变体在艾滋病发展之前，始终会在第一个可变区（V1）中进化出多个新的潜在糖基化位点。在本研究中，我们探究了带有这些糖基化位点的病毒在传播给未感染的猕猴后是否会持续存在。将传播给受体猕猴后进化出的变体与在供体中进化的病毒进行比较，供体已被克隆的SIV Mne感染。传播后，V1中潜在编码新的O-连接糖基化位点的特定富含丝氨酸/苏氨酸的基序在从受体的淋巴结、脾脏和肝脏组织中分离出的病毒中得以保留。受体病毒的包膜V3区有一些变化积累，而供体猕猴的病毒中未观察到该区域的变化。尸检时在受体组织中检测到的一些变体与供体接种物中存在的病毒关系最为密切，尽管这些特定变体在受体外周血单核细胞感染后早期未被检测到。总体而言，具有与疾病进展相关的主要V1序列的病毒会传播到受体动物并在其中持续存在。
