[Comparison of numbers of active lymphocytes B in spleen of mice infected with Trichinella pseudospiralis or Trichinella spiralis].

The experiments were performed on BALB/c mice (male, 20 g body weight). The mice were infected 23 days before the experiment by peroral application of about 100 Trichinella larves. Control group consisted of not infected mice. The number of lymphocytes B producing antibodies was determined according to Cunningham and Szenberg. Five days before performance of the test, all mice were immunized by peritoneal application of sheep erythrocyte suspension. After that the mice were sacrificed, spleen was isolated and cellular suspension was prepared which was then introduced to the chamber in presence of sheep erythrocytes and guinea pig complement. After one hour incubation clearance appearing around active lymphocytes B was counted. Results were expressed in percentages, accepting as 100% number of clearances in not infected mice. It was found that number of clearances was reduced to 40% in mice infected with Trichinella pseudospiralis and to 46% in animals infected with Trichinella spiralis. Similar results were obtained in mice to whom lipid fraction of Trichinella extract containing prostaglandins was applied. These results indicate immunosuppressive influence of invasion on mice organism and also on the role of prostaglandins in evoking this immunosuppression.