Ishikawa T, Mitsuhashi M, Ichikawa Y, Tarnawski A
DVA Medical Center, Long Beach.
Life Sci. 1994;54(18):1329-34. doi: 10.1016/0024-3205(94)00511-7.
Effect of epidermal growth factor (EGF) on ornithine decarboxylase (ODC) was examined in human gastric cancer-derived KATO-III cells, because 125I-EGF binding studies indicated a presence of specific binding sites for EGF on these cells. Upon stimulation with EGF, both ODC mRNA expression and ODC enzyme activity were significantly increased in KATO-III cells. However, unlike in other cellular systems, both EGF-induced ODC mRNA expression and ODC enzyme activation were biphasic with the peaks at 15 +/- 10 min and 2.1 +/- 1.5 hrs (mean +/- SE) for mRNA, and 3.1 +/- 1.5 and 7.7 +/- 1.8 hrs (mean +/- SE) for enzyme activity, respectively. Therefore, KATO-III cell line may provide a unique model for the biochemical analysis of EGF action on ODC activation.
在人胃癌来源的KATO-III细胞中检测了表皮生长因子（EGF）对鸟氨酸脱羧酶（ODC）的影响，因为125I-EGF结合研究表明这些细胞上存在EGF的特异性结合位点。用EGF刺激后，KATO-III细胞中的ODC mRNA表达和ODC酶活性均显著增加。然而，与其他细胞系统不同，EGF诱导的ODC mRNA表达和ODC酶激活都是双相的，mRNA的峰值分别在15±10分钟和2.1±1.5小时（平均值±标准误），酶活性的峰值分别在3.1±1.5小时和7.7±1.8小时（平均值±标准误）。因此，KATO-III细胞系可能为EGF对ODC激活作用的生化分析提供一个独特的模型。
