Inglis W L, Allen L F, Whitelaw R B, Latimer M P, Brace H M, Winn P
School of Psychology, University of St Andrews, Fife, U.K.
Neuroscience. 1994 Feb;58(4):817-33. doi: 10.1016/0306-4522(94)90459-6.
As the pedunculopontine tegmental nucleus has an important anatomical position as an output station for the striatum, its role in the mediation of behaviour stimulated by d-amphetamine and apomorphine was investigated. Bilateral ibotenate lesions were made in either the pedunculopontine tegmental nucleus or, as a control, in the adjacent deep mesencephalic nucleus; sham lesions were made using phosphate buffer. Over the 14 days after surgery there were no significant differences in the rats' body weight or food intake. Deep mesencephalic lesioned rats spilled more food and drank more water (never more than 5 ml more) than controls or pedunculopontine tegmental lesioned rats. Spontaneous locomotion and that elicited by d-amphetamine or apomorphine were not affected by ibotenate lesions of either the pedunculopontine tegmental nucleus or deep mesencephalic nucleus. At higher doses of d-amphetamine and apomorphine, however, excessive biting and licking were observed in the pedunculopontine tegmental nucleus, but not deep mesencephalic nucleus, lesioned rats. Such orofacial stereotypies are never observed in normal rats after systemic injection of d-amphetamine. Post mortem analysis showed that ibotenate lesions of the pedunculopontine tegmental nucleus had destroyed cholinergic and non-cholinergic neurons there but had left the deep mesencephalic nucleus intact; ibotenate lesions of the deep mesencephalic nucleus destroyed neurons in that structure but not the pedunculopontine tegmental nucleus. These data demonstrate that lesions in the pedunculopontine tegmental nucleus and deep mesencephalic nucleus have different effects, measured histologically and behaviourally; that neither spontaneous locomotion nor that stimulated by d-amphetamine or apomorphine is dependent on the integrity of the pedunculopontine tegmental nucleus; and that the pedunculopontine tegmental nucleus plays an important role in mediating orofacial activity stimulated by these drugs. The data are discussed in terms of their implications for understanding outflow from the caudate-putamen and nucleus accumbens.
由于脚桥被盖核作为纹状体的输出站具有重要的解剖学位置，因此研究了其在介导由右旋苯丙胺和阿扑吗啡刺激的行为中的作用。在脚桥被盖核或作为对照的相邻中脑深核中进行双侧鹅膏蕈氨酸损伤；使用磷酸盐缓冲液制作假损伤。在手术后的14天内，大鼠的体重或食物摄入量没有显著差异。中脑深核损伤的大鼠比对照组或脚桥被盖核损伤的大鼠洒出更多食物且饮水更多（从不超过多5毫升）。脚桥被盖核或中脑深核的鹅膏蕈氨酸损伤均不影响自发运动以及由右旋苯丙胺或阿扑吗啡引发的运动。然而，在较高剂量的右旋苯丙胺和阿扑吗啡作用下，脚桥被盖核损伤的大鼠出现过度咬和舔的行为，而中脑深核损伤的大鼠则未出现。在正常大鼠全身注射右旋苯丙胺后从未观察到这种口面部刻板行为。死后分析表明，脚桥被盖核的鹅膏蕈氨酸损伤破坏了那里的胆碱能和非胆碱能神经元，但中脑深核完好无损；中脑深核的鹅膏蕈氨酸损伤破坏了该结构中的神经元，但未损伤脚桥被盖核。这些数据表明，脚桥被盖核和中脑深核的损伤在组织学和行为学测量上具有不同的影响；自发运动以及由右旋苯丙胺或阿扑吗啡刺激的运动均不依赖于脚桥被盖核的完整性；并且脚桥被盖核在介导这些药物刺激的口面部活动中起重要作用。根据这些数据对理解尾状核 - 壳核和伏隔核流出的意义进行了讨论。
