Marquette C, Van Dam A M, Van Rooijen N, Berkenbosch F, Haour F
Unité de Pharmacologie Neuro-Immuno-Endocrinienne, Institut Pasteur, France.
Psychoneuroendocrinology. 1994;19(2):189-96. doi: 10.1016/0306-4530(94)90008-6.
Interleukin-1 receptors (IL-1R) have been characterized in the brain and pituitary gland of mice. Previous studies have demonstrated that following lipopolysaccharide (LPS) injection, IL-1R density decreases in the dentate gyrus and in the choroid plexus. Receptors present in the anterior pituitary gland remain unchanged under the same experimental conditions. In this study, we investigated the role of peripheral macrophages in LPS-induced downregulation of IL-1 receptors. Mice were injected with liposomes encapsulated with dichloromethylene diphosphonate (Cl2MDP), which induced a profound depletion of peripheral macrophages. Immunocytochemistry was used to determine the efficiency of macrophage elimination. Depletion of macrophages did not affect the density of central and pituitary IL-1R in non-LPS-challenge mice. However, the liposome treatment prevented downregulation of IL-1R in the dentate gyrus observed following LPS administration. In addition, LPS induced a slight decrease in IL-1R density in the choroid plexus but not in the anterior pituitary gland of liposome treated mice. These results suggest that peripheral macrophages play an important role in the LPS-induced modulation of central IL-1R.
白细胞介素-1受体（IL-1R）已在小鼠的脑和垂体中得到表征。先前的研究表明，注射脂多糖（LPS）后，齿状回和脉络丛中的IL-1R密度降低。在相同实验条件下，垂体前叶中的受体保持不变。在本研究中，我们调查了外周巨噬细胞在LPS诱导的IL-1受体下调中的作用。给小鼠注射包裹有二氯亚甲基二膦酸盐（Cl2MDP）的脂质体，这导致外周巨噬细胞大量耗竭。免疫细胞化学用于确定巨噬细胞清除的效率。巨噬细胞的耗竭不影响未受LPS攻击的小鼠中枢和垂体IL-1R的密度。然而，脂质体处理可防止LPS给药后齿状回中IL-1R的下调。此外，LPS使脂质体处理小鼠的脉络丛中IL-1R密度略有降低，但垂体前叶中未出现这种情况。这些结果表明，外周巨噬细胞在LPS诱导的中枢IL-1R调节中起重要作用。
