Dubois D H, Smith L C, Bazer F W
Animal Science Department, University of Florida, Gainesville 32611.
Reprod Fertil Dev. 1993;5(5):531-43. doi: 10.1071/rd9930531.
Experiments examined the characteristics and activity of phospholipase A2 (PLA2) and examined the presence of immunoreactive cyclooxygenase in endometrium of pigs during the oestrous cycle and early pregnancy. Endometrial PLA2 was calcium-independent and activity of the enzyme was greatest at a pH of 8.0. Activity of PLA2 on Days 10, 12, 14 and 16 of the oestrous cycle did not differ (P > 0.1) from activity on those days during pregnancy. During oestrus and early metoestrus (Days 0-3), cyclooxygenase was present in both glandular and surface epithelium. After Day 10 of the oestrous cycle or pregnancy, staining for cyclooxygenase was less intense in the lower and middle uterine glands. However, the upper glandular epithelium near the surface epithelium stained intensely. By Day 15 of the oestrous cycle or pregnancy, intense staining for cyclooxygenase appeared restricted to the upper uterine glands. These results indicate changes in localization of immunoreactive cyclooxygenase throughout the oestrous cycle and suggest that these are not related to altered secretion of prostaglandins (PGs) during early pregnancy. The stimulatory effects of porcine conceptus products on secretion of PGs during early pregnancy are apparently not associated with increased activity of endometrial PLA2.
实验研究了磷脂酶A2（PLA2）的特性和活性，并检测了发情周期和妊娠早期猪子宫内膜中免疫反应性环氧化酶的存在情况。子宫内膜PLA2不依赖钙，该酶在pH值为8.0时活性最强。发情周期第10、12、14和16天的PLA2活性与妊娠期间这些日子的活性无差异（P>0.1）。在发情期和发情后期早期（第0 - 3天），腺上皮和表面上皮均存在环氧化酶。发情周期或妊娠第10天后，子宫下部和中部腺体中环氧化酶的染色强度减弱。然而，靠近表面上皮的上部腺上皮染色强烈。到发情周期或妊娠第15天，环氧化酶的强烈染色似乎仅限于子宫上部腺体。这些结果表明，在整个发情周期中免疫反应性环氧化酶的定位发生了变化，并表明这些变化与妊娠早期前列腺素（PGs）分泌的改变无关。妊娠早期猪胚胎产物对PGs分泌的刺激作用显然与子宫内膜PLA2活性增加无关。
