Du Z F, Wales R G
School of Veterinary Studies, Murdoch University, Australia.
Reprod Fertil Dev. 1993;5(5):555-65. doi: 10.1071/rd9930555.
The oxidation and incorporation of glucose and glutamine by embryos derived from cultured zygotes was compared with the utilization of these substrates by embryos recovered directly from the reproductive tract of pregnant females. The oxidation of glutamine was greater at the blastocyst stage than at the 2-cell stage. Embryos derived from outbred females (Qs) were less active in the oxidation of glutamine than those from hybrid (B10D2F1) females and development in culture was detrimental to this oxidation, especially in blastocysts from the outbred stock. The oxidation of glutamine was stimulated by the presence of glucose at the 2-cell stage but reduced by its presence at the blastocyst stage. Maternal genotype had no effect on the oxidation of glucose at either the 2-cell or blastocyst stage, and only at the blastocyst stage was there evidence of a detrimental effect of culture. The oxidation of glucose was stimulated by the presence of glutamine at the 2-cell stage but depressed by its addition at the blastocyst stage. Incorporation of glutamine increased with development, but this was reduced at the blastocyst stage by development in culture, especially if the blastocysts were derived from outbred females. Incorporation of glucose also increased with development. At the 2-cell stage, culture reduced incorporation of this substrate, especially into the acid-soluble fraction of embryos from outbred females. In blastocysts, incorporation of glucose into the acid-insoluble fraction was depressed by culture and in embryos from outbred females. In contrast to glucose oxidation, incorporation of glucose into the acid-soluble fraction was reduced by the addition of glutamine at the 2-cell stage but increased by its addition at the blastocyst stage.
将培养的受精卵发育而来的胚胎对葡萄糖和谷氨酰胺的氧化及摄取情况，与直接从怀孕雌性生殖道回收的胚胎对这些底物的利用情况进行了比较。谷氨酰胺的氧化在囊胚期比在2细胞期更强烈。远交雌性（Qs）来源的胚胎在谷氨酰胺氧化方面的活性低于杂种（B10D2F1）雌性来源的胚胎，且体外培养对这种氧化有不利影响，尤其是远交品系来源的囊胚。在2细胞期，葡萄糖的存在会刺激谷氨酰胺的氧化，但在囊胚期则会使其氧化减少。母体基因型在2细胞期或囊胚期对葡萄糖的氧化均无影响，仅在囊胚期有证据表明培养有不利影响。在2细胞期，谷氨酰胺的存在会刺激葡萄糖的氧化，但在囊胚期添加谷氨酰胺则会使其氧化受到抑制。谷氨酰胺的摄取随发育而增加，但在囊胚期，体外培养会使其摄取减少，尤其是当囊胚来自远交雌性时。葡萄糖的摄取也随发育而增加。在2细胞期，培养会减少这种底物的摄取，尤其是远交雌性胚胎中进入酸溶性部分的摄取。在囊胚中，培养会抑制葡萄糖进入酸不溶性部分的摄取，远交雌性胚胎的情况也是如此。与葡萄糖氧化相反，在2细胞期添加谷氨酰胺会减少葡萄糖进入酸溶性部分的摄取，但在囊胚期添加则会增加这种摄取。
