[Immunologic analysis of the variations of hemolymph proteins of Astacus leptodactylus during the premolt].

The variations in the hemolymph proteins of crayfish were studied by quantitative protein determination, elctrophoresis on polyacrylamide gel gradient and tandem crossed immuno-electrophoresis, from stage C 4 to the end of stage D. The total protein concentration varies during the premolt, with two peaks in D 0 and D I'''-D2, but the plasmatic "fibrinogen" rate is about the same during this period. But three fractions exist which show important changes during the molt cycle.