Dejours P, Armand J
Respir Physiol. 1980 Jul;41(1):1-11. doi: 10.1016/0034-5687(80)90019-5.
The acid-base balance of the prebranchial hemolymph of the crayfish Astacus leptodactylus was studied at various acid-base balances and levels of oxygenation of the ambient water at 13 degrees C. The water acid-base balance was controlled automatically by a pH-CO2-stat. Into water of constant titration alkalinity, TA, this device intermittenly injects carbon dioxide to maintain the pH at a preset value. Water pH was reduced to the same value either by hypercapnia (at constant TA) or by adding HCl or H2SO4 to decrease the TA (at constant CO2 tension). Decrease of hemolymph pH and increase of hemolymph PCO2 were similar for the three acidic waters. Water oxygenation changes strongly affected hemolymph ABB. In crayfish living in hyperoxic water (PO2 congruent to 600 Torr) compared to those in hypoxic water (PO2 congruent to 40 Torr), hemolymph pH was 0.3 to 0.4 unit lower and hemolymph PCO2 several times higher, the exact values of pH and PCO2 depending on the controlled ambient acid-base balance. In any study of the hemolymph acid-base balance of the crayfish, it is an important to control ambient water's acid-base balance and oxygenation as it is to control its temperature, a conclusion which probably holds true for studies on all water breathers.
在13摄氏度下，研究了细足螯虾鳃前血淋巴的酸碱平衡，实验设置了不同的酸碱平衡和环境水氧合水平。水的酸碱平衡由pH-CO₂控制器自动控制。该装置向具有恒定滴定碱度（TA）的水中间歇性注入二氧化碳，以将pH维持在预设值。通过高碳酸血症（在恒定TA下）或添加HCl或H₂SO₄以降低TA（在恒定CO₂张力下），将水的pH降低到相同值。三种酸性水导致的血淋巴pH降低和血淋巴PCO₂升高情况相似。水氧合变化对血淋巴酸碱平衡有强烈影响。与生活在低氧水中（PO₂约为40 Torr）的小龙虾相比，生活在高氧水中（PO₂约为600 Torr）的小龙虾，其血淋巴pH低0.3至0.4个单位，血淋巴PCO₂高出数倍，pH和PCO₂的确切值取决于受控的环境酸碱平衡。在任何关于小龙虾血淋巴酸碱平衡的研究中，控制环境水的酸碱平衡和氧合与控制其温度一样重要，这一结论可能适用于所有水生呼吸动物的研究。
