Ehinger B, Lindberg-Bauer B
Brain Res. 1976 Sep 3;113(3):535-49. doi: 10.1016/0006-8993(76)90055-x.
The light-evoked release of [3H]glycine from retina in cat (in vivo) after pre-retinal perfusion and in rabbit (in vitro) after intravitreal injection was studied. The site of uptake of [3H]glycine into retina was checked by autoradiography and was found to be almost exclusively in a type of amacrine cells. If the retina loaded with [3H]glycine was stimulated by light flashes the release increased significantly in both in vivo and in vitro experiments. When the flashing light was exchanged for continuous light there was not change in the spontaneous efflux of radioactivity. Chromatographic experiments showed that the main part of the radioactivity released by light was glycine. The light-evoked release of glycine from retina was dependent on temperature and Ca2+. Low temperature (+2 to +4 degrees C) abolished the increased release. If Ca2+ was ommitted from the perfusion medium and EDTA was added there was no light-inducable change in the efflux of radioactivity in retinas loaded with [3H]valine. The present results, that light stimulation will release glycine from the retina both in vivo and in vitro, are further criterion for it as neurotransmitter.
研究了在猫体内视网膜前灌注后以及兔体外玻璃体内注射后，光诱发的视网膜中[3H]甘氨酸的释放情况。通过放射自显影检查了[3H]甘氨酸进入视网膜的摄取部位，发现几乎完全位于一种无长突细胞类型中。如果用闪光刺激加载有[3H]甘氨酸的视网膜，在体内和体外实验中释放均显著增加。当闪光换成持续光时，放射性的自发流出没有变化。色谱实验表明，光释放的放射性主要部分是甘氨酸。视网膜中光诱发的甘氨酸释放依赖于温度和Ca2+。低温（+2至+4摄氏度）消除了释放的增加。如果从灌注培养基中省略Ca2+并添加EDTA，加载有[3H]缬氨酸的视网膜中放射性流出没有光诱导变化。目前的结果，即光刺激在体内和体外均会从视网膜释放甘氨酸，是其作为神经递质的进一步标准。
