Sasaki K, Pabst R, Rothkötter H J
Department of Anatomy, Yokohama City University School of Medicine, Japan.
Anat Rec. 1994 Apr;238(4):473-9. doi: 10.1002/ar.1092380406.
Vascular reconstruction during rat lymph node regeneration was investigated in autotransplanted mesenteric lymph node fragments, which had been implanted in the renal parenchyma. In addition to light microscopy, vascular casting and transmission electron microscopy were used. From day 3 onwards capillaries grew into the autografts together with lymphatic vessels. The capillaries showed obvious signs of proliferation by day 5. The surviving interstitial cells at the outer border of the transplant produced extracellular substance. High endothelial venules (HEV) differentiated from capillaries from about day 7. A first sign of their development was a vessel with a narrow, branching luminal space and with endothelial cells containing rich cytoplasm and small Golgi complexes. As the Golgi complexes grew and the cisternae and vesicles increased, the lumen dilated, the cell coat on the luminal surface became prominent, and, finally, lymphocytes emigrated through these venules from around day 10. The typical lymph node structure was complete by day 28. These results suggest that the interaction among the remaining interstitial cells, invading capillaries, and lymphatic penetration results in differentiation and maturation of HEV in lymph node regeneration. The development of Golgi complexes is strongly associated with lymphocyte emigration from the blood.
对自体移植到肾实质的肠系膜淋巴结片段进行大鼠淋巴结再生过程中的血管重建研究。除了光学显微镜检查外，还使用了血管铸型和透射电子显微镜检查。从第3天起，毛细血管与淋巴管一起长入自体移植物。到第5天，毛细血管显示出明显的增殖迹象。移植体外缘存活的间质细胞产生细胞外物质。大约从第7天起，高内皮微静脉（HEV）从毛细血管分化而来。其发育的第一个迹象是一个血管，其管腔狭窄、分支，内皮细胞含有丰富的细胞质和小的高尔基体复合体。随着高尔基体复合体的生长以及扁平囊和小泡数量的增加，管腔扩张，腔表面的细胞衣变得突出，最终，大约从第10天起淋巴细胞通过这些微静脉迁移。到第28天，典型的淋巴结结构完整。这些结果表明，剩余的间质细胞、侵入的毛细血管和淋巴渗透之间的相互作用导致了淋巴结再生过程中HEV的分化和成熟。高尔基体复合体的发育与淋巴细胞从血液中的迁移密切相关。
