Gauthier E R, Dumas C, Chapdelaine P, Tremblay R R, Dubé J Y
Hormonal Bioregulation Laboratory, CHUL Research Center, Ste-Foy, Quebec, Canada.
Biochim Biophys Acta. 1994 May 17;1218(1):102-4. doi: 10.1016/0167-4781(94)90108-2.
Using a combination of primer extension and RT-PCR, the cDNA encoding a canine tissue kallikrein expressed in the pancreas was cloned and sequenced. The cloned 0.85 kbp cDNA contained a complete open reading frame encoding a polypeptide of 261 amino acids. The calculated molecular mass of the processed, unglycosylated, 237 amino acid protein was 26,428 Da. Its mRNA was expressed at high levels in the pancreas, kidney and submaxillary gland. The sequence of the encoded protein was highly homologous with canine prostatic arginine esterase (66%) and human renal/pancreatic kallikrein (74%). Therefore, the cloned cDNA encoded a previously uncharacterized canine kallikrein enzyme which was named dog renal/pancreatic kallikrein or dK2 according to the new nomenclature for kallikrein gene family members. Because of its specific pattern of tissue expression and the presence of all the amino acid residues necessary for kininogenase activity, we suggest that dK2 is the canine true tissue kallikrein.
运用引物延伸和逆转录聚合酶链反应（RT-PCR）相结合的方法，克隆并测序了在犬胰腺中表达的一种犬组织激肽释放酶的编码cDNA。克隆得到的0.85 kbp cDNA包含一个完整的开放阅读框，编码一个由261个氨基酸组成的多肽。经计算，加工后的、未糖基化的由237个氨基酸组成的蛋白质分子量为26,428 Da。其mRNA在胰腺、肾脏和颌下腺中高水平表达。所编码蛋白质的序列与犬前列腺精氨酸酯酶高度同源（66%），与人肾/胰腺激肽释放酶高度同源（74%）。因此，克隆得到的cDNA编码一种以前未被鉴定的犬激肽释放酶，根据激肽释放酶基因家族成员的新命名法，将其命名为犬肾/胰腺激肽释放酶或dK2。由于其特定的组织表达模式以及存在激肽原酶活性所需的所有氨基酸残基，我们认为dK2是犬真正的组织激肽释放酶。
