Satoh T, Kobayashi M, Kaneda M, Tanihiro M, Okada K, Ueda K
Department of Pediatrics, Hiroshima University School of Medicine, Japan.
Blood. 1994 Jun 1;83(11):3312-5.
We classified the genotype of neutrophil Fc gamma receptor III (FcRIII) (CD16) with a new method. Genomic DNA from mononuclear cells of 39 unrelated healthy donors (13 NA1/NA1, 13 NA2/NA2, and 13 NA1/NA2 typed serologically) were subjected to polymerase chain reaction (PCR) to amplify the polymorphic third exon of the FcRIII genes. The PCR products were heat denatured, electrophoresed, and visualized by silver staining. Allelic differences were detected by distinctive electrophoretic patterns of each single strand, depending on their sequence specific conformations (single-strand conformation polymorphism [SSCP]). The genotypes of neutrophil FcRIII determined by this method were consistent with the phenotypes of NA antigens determined by serologic examinations. These results indicate that the PCR-SSCP system is a very useful tool for genotyping of the neutrophil FcRIII.
我们用一种新方法对中性粒细胞Fcγ受体III（FcRIII，CD16）的基因型进行了分类。对39名无亲缘关系的健康供者（血清学检测13例NA1/NA1、13例NA2/NA2和13例NA1/NA2）单核细胞的基因组DNA进行聚合酶链反应（PCR），以扩增FcRIII基因的多态性第三外显子。PCR产物经热变性、电泳，并通过银染显色。根据各单链独特的电泳图谱检测等位基因差异，这取决于它们的序列特异性构象（单链构象多态性[SSCP]）。通过该方法确定的中性粒细胞FcRIII基因型与血清学检测确定的NA抗原表型一致。这些结果表明，PCR-SSCP系统是中性粒细胞FcRIII基因分型的非常有用的工具。
