Tonevitsky A G, Agapov I I, Mechetner E B, Ershova G V, Sarma T, Shamshiev A T, Pfueller U
Institute for Genetics and Selection of Industrial Microorganisms, Moscow, Russia.
Biochem Mol Biol Int. 1993 Dec;31(6):1059-69.
In comparative experiments the conjugates of A-chain of a plant toxin ricin (RTA) and monoclonal antibody (MAb) HAE9 (IgM) directed against human erythroblast antigen (Ag-Eb) or Mab HAE3 against human glycophorin-A and immunotoxins (IT) directed to CD5 and CD7 antigens of human T-lymphocytes have been investigated. Proceeding from the number of receptors on a cell, we compared efficiency of the cytotoxic effects of the conjugates with different internalization rate. The efficiency of immunoconjugates against Ags with an extremely high internalization rate was only slightly higher than that of immunoconjugates against Ags with a lower internalization rate. The enhancing effect of ammonium chloride on immunoconjugate cytotoxicity was more pronounced in the case of immunotoxins with a high internalization rate.
在比较实验中，研究了植物毒素蓖麻毒素（RTA）A链与针对人成红细胞抗原（Ag-Eb）的单克隆抗体（MAb）HAE9（IgM）或针对人血型糖蛋白A的MAb HAE3的缀合物，以及针对人T淋巴细胞CD5和CD7抗原的免疫毒素（IT）。根据细胞上受体的数量，我们比较了具有不同内化速率的缀合物的细胞毒性作用效率。针对具有极高内化速率的抗原的免疫缀合物的效率仅略高于针对具有较低内化速率的抗原的免疫缀合物。氯化铵对免疫缀合物细胞毒性的增强作用在具有高内化速率的免疫毒素的情况下更为明显。
