Patarnello T, Bargelloni L, Caldara F, Colombo L
Department of Biology, University of Padova, Italy.
Mol Mar Biol Biotechnol. 1993 Dec;2(6):333-7.
PCR and direct sequencing of 300 to 377-bp fragments of the cytochrome b mitochondrial gene were carried out in 40 specimens of European sea bass, Dicentrarchus labrax L., collected at different sites in the Mediterranean basin and representing wild and farmed populations. Sequence analysis revealed 6 transitional changes, which generated 5 different haplotypes (A-E). Only one mutation was nonsynonymous because it occurred in the first position of a codon leading to the replacement of valine by isoleucine. The genotypic distribution revealed that haplotype B was not present in the farm samples, whereas its frequency in the wild samples exceeded 30%. In contrast, haplotypes D and E were found in farmed populations but not in the wild populations. The comparison among wild populations showed a nonhomogeneous distribution of the two most frequent haplotypes, A and B.
对40个欧洲海鲈样本（欧洲鲈，Dicentrarchus labrax L.）的细胞色素b线粒体基因300至377bp片段进行了聚合酶链反应（PCR）和直接测序，这些样本采集于地中海盆地的不同地点，代表野生和养殖群体。序列分析揭示了6个转换变化，产生了5种不同的单倍型（A - E）。只有一个突变是非同义的，因为它发生在密码子的第一位，导致缬氨酸被异亮氨酸取代。基因型分布显示，养殖样本中不存在单倍型B，而其在野生样本中的频率超过30%。相反，单倍型D和E在养殖群体中发现，但在野生群体中未发现。野生群体之间的比较显示，两种最常见的单倍型A和B分布不均匀。
