Gruft H, Loder A, Osterhout M
Health Lab Sci. 1976 Jul;13(3):184-9.
The New York State Department of Health proficiency testing program in mycobacteriology determines the ability of a laboratory to isolate the acid-fast bacillus present in a simulated specimen and to identify the strain. Until 1964 the specimens were autoclaved normal sputa seeded with mycobacteria. When mandatory testing was introduced by law in 1964, more specimens were needed. These have been prepared in simulated sputum bases, first skimmed milk, then granulated hog gastric mucin. The bases are seeded with Mycobacterium tuberculosis and other mycobacteria; Pseudomonas aeruginosa is added to simulate the contamination in clinical specimens. Laboratory performance in general has improved as a result of the proficiency testing and the concomitant educational program, but laboratories processing more than 200 specimens a year continue to perform best.
纽约州卫生部的分枝杆菌学能力验证计划，旨在确定实验室从模拟标本中分离出抗酸杆菌并鉴定菌株的能力。1964年以前，标本是接种了分枝杆菌的经高压灭菌的正常痰液。1964年法律规定实行强制性检测后，需要更多标本。这些标本是在模拟痰液基质中制备的，最初是脱脂牛奶，然后是颗粒状猪胃粘蛋白。基质中接种了结核分枝杆菌和其他分枝杆菌；添加铜绿假单胞菌以模拟临床标本中的污染情况。由于能力验证和随之开展的教育计划，实验室的总体表现有所改善，但每年处理超过200份标本的实验室仍然表现最佳。
