Xu J, Wang N
Department of Pediatrics, University of Rochester School of Medicine and Dentistry, New York 14642.
Cancer Genet Cytogenet. 1994 May;74(1):1-7. doi: 10.1016/0165-4608(94)90020-5.
In order to identify chromosomal structural alterations in the ovarian carcinoma cell line MLS/P, fluorescence in situ hybridization with centromeric probes for chromosomes 1, 8, 9, 13/21, 14/22, 15, 17, and X and whole chromosome painting probes for chromosomes 1, 3, 4, 5, 7, 8, 9, 10, 12, 13, 14, 17, 19, 22, and X were performed subsequent to GTG-banding. This combined approach identified 14 of the 18 clonal structurally rearranged chromosomes, with the X chromosome involved in three aberrations. In contrast, only eight of the 14 rearrangements were identifiable by G-banding alone. These results indicate that the combined G-banding and FISH approach can significantly improve the cytogenetic analysis of human neoplasia.
为了鉴定卵巢癌细胞系MLS/P中的染色体结构改变，在GTG显带之后，使用针对1号、8号、9号、13/21号、14/22号、15号、17号和X染色体的着丝粒探针以及针对1号、3号、4号、5号、7号、8号、9号、10号、12号、13号、14号、17号、19号、22号和X染色体的全染色体涂染探针进行荧光原位杂交。这种联合方法鉴定出了18条发生克隆性结构重排染色体中的14条，其中X染色体涉及3种畸变。相比之下，仅通过G显带只能鉴定出14种重排中的8种。这些结果表明，G显带和荧光原位杂交联合方法能够显著改善人类肿瘤的细胞遗传学分析。
