Takayanagi T, Kimura A, Chiba S, Ajisaka K
Meiji Institute of Health Science, Meiji Milk Products Co., Ltd., Odawara, Japan.
Carbohydr Res. 1994 Mar 18;256(1):149-58. doi: 10.1016/0008-6215(94)84234-5.
Seven oligosaccharides were isolated from alpha-D-glucosidase (EC 3.2.1.20) from Aspergillus niger, and the structures of these oligosaccharides were studied by 1H NMR spectroscopy. After treatment of the alpha-D-glucosidase with N-glycosidase F, seven major oligosaccharide peaks were detected by Dionex anion-exchange HPLC. The structures corresponding to the three peaks OS-1, OS-2, and OS-4 were determined to be Man8GlcNAc2, Man9GlcNAc2, and GlcMan9GlcNAc2, respectively, from 1H NMR spectra of the isolated fractions. Each of the four oligosaccharides OS-5, OS-6, OS-7-1, and OS-7-2 contained an alpha-D-galactofuranosyl residue (Galf) linked to Man(A) via an alpha-(1-->2)-linkage. OS-7 was found to consist of two oligosaccharides. The structures of these four oligosaccharides were determined to be GalfMan5GlcNAc2, GalfMan6GlcNAc2, GalfMan7GlcNAc2, and GalfMan8GlcNAc2 by 1H NMR spectroscopy and compositional analysis. The Galf structure of GalfMan5GlcNAc2 was found to be identical to that of an oligosaccharide previously isolated from the alpha-D-galactosidase of the same strain. The structure of OS-3 remains undetermined.
从黑曲霉的α-D-葡萄糖苷酶（EC 3.2.1.20）中分离出7种寡糖，并通过1H NMR光谱研究了这些寡糖的结构。用N-糖苷酶F处理α-D-葡萄糖苷酶后，通过戴安离子交换高效液相色谱法检测到7个主要的寡糖峰。根据分离组分的1H NMR光谱，确定与三个峰OS-1、OS-2和OS-4相对应的结构分别为Man8GlcNAc2、Man9GlcNAc2和GlcMan9GlcNAc2。四种寡糖OS-5、OS-6、OS-7-1和OS-7-2中的每一种都含有一个通过α-(1→2)-连接与Man(A)相连的α-D-呋喃半乳糖基残基（Galf）。发现OS-7由两种寡糖组成。通过1H NMR光谱和组成分析确定这四种寡糖的结构分别为GalfMan5GlcNAc2、GalfMan6GlcNAc2、GalfMan7GlcNAc2和GalfMan8GlcNAc2。发现GalfMan5GlcNAc2的Galf结构与先前从同一菌株的α-D-半乳糖苷酶中分离出的一种寡糖的结构相同。OS-3的结构尚未确定。
