Differentiation markers for lung-cancer sub-types. A comparative study of their expression in vivo and in vitro.

Cell lines representing the major sub-types of lung cancer have proved to be useful tools to study the molecular and cellular biology of these malignancies, provided that they are well established and well characterized. Antibodies directed against constituents of different cellular compartments can detect the type and degree of differentiation in lung cancer and derived cell lines. Antibodies can detect cell-surface adhesion molecules, such as NCAM, cadherins and integrins. NCAM antibodies are able to differentiate between small-cell lung cancer (SCLC) and non-SCLC, both in cell lines and in tumours. In addition, a spectrum of other membrane proteins, expressed in solid tumours, such as epidermal-growth-factor receptor and carcino-embryonic antigen, are retained in cell lines. Cytoplasmic intermediate filament proteins appear to be generally retained in lung-cancer cell lines, their combinations being the same as in solid SCLC, adenocarcinomas and squamous-cell carcinomas. Nuclear expression of lamins is comparable in tumours and in their corresponding cell lines and can be used to differentiate between SCLC and non-SCLC: A-type lamins, which are present in non-SCLC, are absent in most SCLC.