[人促甲状腺激素释放激素受体的克隆与特性：甲状腺激素对促甲状腺激素释放激素受体mRNA水平的调节]

[Cloning and characterization of the human thyrotropin-releasing hormone receptor: regulation of thyrotropin-releasing hormone receptor mRNA levels by thyroid hormone].

Yamada M, Mori M

First Department of Internal Medicine, Gunma University, School of Medicine.

Nihon Rinsho. 1994 Apr;52(4):1001-6.

Abstract

We successfully cloned a human thyrotropin-releasing hormone receptor (TRH-R) gene. The human TRH-R consists of 398 amino acids and is a member of the G protein-coupled receptor family. The gene has a single intron in the coding sequence. Although highly homologous to the rat and mouse TRH-Rs, the human TRH-R has several differences in the carboxyl-terminal structure. 2) We next studied the effect of thyroid status on TRH-R mRNA levels both in vivo and in vitro. TRH-R mRNA levels in hypothyroid rats were increased significantly, while no significant changes were observed in vitro. The data indicate that thyroid hormone may regulate TRH-R mRNA levels in different manners in vitro and in vivo.

摘要

我们成功克隆了人类促甲状腺激素释放激素受体（TRH-R）基因。人类TRH-R由398个氨基酸组成，是G蛋白偶联受体家族的一员。该基因在编码序列中有一个单一内含子。尽管与大鼠和小鼠的TRH-R高度同源，但人类TRH-R在羧基末端结构上有几个差异。2) 接下来，我们研究了甲状腺状态对体内和体外TRH-R mRNA水平的影响。甲状腺功能减退大鼠的TRH-R mRNA水平显著升高，而在体外未观察到显著变化。数据表明，甲状腺激素可能在体内和体外以不同方式调节TRH-R mRNA水平。