Murakami M, Miyashita K, Mori M
First Department of Internal Medicine, Gunma University, School of Medicine.
Nihon Rinsho. 1994 Apr;52(4):1057-62.
Immunogenic regions in human TSH receptor responsible for autoimmune thyroid disease have been studied using synthetic TSH receptor-related peptides. Eight different peptides corresponding to segments of the extracellular domain of TSH receptor were synthesized. Immunoglobulin G (IgG) of patients with Graves' disease significantly bound to peptide #1 (32-56) compared with IgG of control subjects, and IgG of three of eight patients with Graves' disease showed increased binding to peptide #5 (309-337). In contrast, IgG of patients with Hashimoto's thyroiditis significantly bound to peptide #8 (333-359). The binding rates of Hashimoto's IgG to peptide #8 positively correlated with both MCHA and TGHA titers. These results suggest that heterogeneity of binding sites in TSH receptor for autoimmune thyroid disease may be responsible for pathophysiology of hyperthyroidism and hypothyroidism.
利用合成的促甲状腺激素（TSH）受体相关肽段，对人类TSH受体中与自身免疫性甲状腺疾病相关的免疫原性区域进行了研究。合成了对应于TSH受体细胞外结构域片段的8种不同肽段。与对照受试者的免疫球蛋白G（IgG）相比，格雷夫斯病患者的IgG与肽段#1（32 - 56）有显著结合，并且8例格雷夫斯病患者中有3例的IgG与肽段#5（309 - 337）的结合增加。相比之下，桥本甲状腺炎患者的IgG与肽段#8（333 - 359）有显著结合。桥本甲状腺炎患者的IgG与肽段#8的结合率与微细胞毒抗体（MCHA）和甲状腺球蛋白抗体（TGHA）滴度均呈正相关。这些结果表明，TSH受体中自身免疫性甲状腺疾病结合位点的异质性可能是甲状腺功能亢进和甲状腺功能减退病理生理学的原因。
