Maggi E, Bellazzi R, Falaschi F, Frattoni A, Perani G, Finardi G, Gazo A, Nai M, Romanini D, Bellomo G
Department of Internal Medicine, IRCCS Policlinico S. Matteo, University of Pavia, Italy.
Kidney Int. 1994 Mar;45(3):876-83. doi: 10.1038/ki.1994.115.
Since oxidized low-density lipoprotein (LDL) is more atherogenic than native LDL, LDL oxidation was investigated in uremic patients who often develop accelerated atherogenesis. Three groups of uremic patients were studied (10 on predialysis conservative therapy, 11 on repetitive hemodialysis, 13 on peritoneal dialysis) and compared with seventy matched controls. LDL oxidation was evaluated in all patients as: (i) the susceptibility to in vitro oxidation (by measuring the resistance to Cu(++)-induced formation of conjugated dienes), (ii) vitamin E concentration in LDL, and (iii) presence of plasma anti-oxidized LDL antibodies, expressed as the ratio anti-oxLDL/anti-nativeLDL antibodies. The lipid profile was studied in all patients. Vitamin E concentration did not differ between the various groups, although LDL from uremic patients appeared more susceptible to in vitro and in vivo oxidation (as demonstrated by an earlier generation of conjugated dienes and by the presence of an higher antibody ratio) compared to control subjects. Subclass analysis of the different patients revealed that peritoneal dialysis treatment ameliorated the oxidation markers. However, a prolonged dialytic treatment caused a decrease in vitamin E concentration in LDL and increased their susceptibility to oxidation.
由于氧化型低密度脂蛋白（LDL）比天然LDL更具致动脉粥样硬化性，因此对常发生加速动脉粥样硬化的尿毒症患者的LDL氧化情况进行了研究。研究了三组尿毒症患者（10例接受透析前保守治疗，11例接受重复血液透析，13例接受腹膜透析），并与70名匹配的对照组进行比较。对所有患者的LDL氧化情况进行了如下评估：（i）体外氧化易感性（通过测量对Cu(++)诱导的共轭二烯形成的抗性），（ii）LDL中的维生素E浓度，以及（iii）血浆抗氧化型LDL抗体的存在情况，以抗氧化型LDL/抗天然LDL抗体的比率表示。对所有患者的血脂谱进行了研究。尽管与对照组相比，尿毒症患者的LDL在体外和体内似乎更易氧化（表现为共轭二烯生成更早且抗体比率更高），但不同组之间的维生素E浓度并无差异。对不同患者的亚组分析显示，腹膜透析治疗改善了氧化标志物。然而，长期透析治疗导致LDL中维生素E浓度降低，并增加了其氧化易感性。
